paper n a su9 egfp addgene 23214 pcmv6 lrrc8b origene rc205553 pcmv6 lrrc8c origene rc222603 pcmv6 lrrc8e origene rc209849 pires2 swell1 qiu  (Addgene inc)

Journal: Nature Communications

Article Title: Calcium transients regulate the apical emergence of basally located progenitors during Xenopus skin development

doi: 10.1038/s41467-025-61610-7

Figure Lengend Snippet: A Representative MIP image of the apical surface area of a MCC expressing calmodulin-GFP. Calmodulin displays colocalization with the basal bodies and the apical actin network. B Schematic depicting the localization of calmodulin in a MCC in relation to the basal bodies and the apical actin network. C Representative images of the skin epithelium from stage 24 control and calmodulin inhibitor-treated embryos. MCC fail to fully integrate into the superficial skin epithelium when calmodulin activity is blocked, as evident by their small apical surface area. D Quantification of the apical surface area of MCCs from control ( n = 100 MCCs) and calmodulin inhibitor-treated embryos ( n = 100 MCCs), 5 different embryos. Two-sided unpaired student’s t test; **** p < 0.0001; mean ± SEM. E Representative images of the skin neuroepithelium from embryos expressing wild-type calmodulin or Ca 2+ binding deficient calmodulin mutant (CALM1234). Expression of CALM1234 results in defective MCC apical emergence (green arrows) while expression of WT calmodulin does not affect MCC apical emergence (white arrows). F Quantification of MCC apical emergence upon expression of WT and Ca 2+ binding deficient mutant calmodulin. Two-sided χ 2 test; ** * * p < 0.0001. G Quantification of the apical surface area of MCCs expressing WT and Ca 2+ binding deficient mutant calmodulin. N = 80 control MCCs, 46 MCCs expressing WT calmodulin and 20 MCCs expressing mutant calmodulin from 4 embryos injected with WT calmodulin DNA and 4 embryos injected with mutant CALM1234 DNA. Two-sided unpaired student’s t test; **** p < 0.0001; mean ± SEM. Scale bars: A : 2 μm, D , F : 20 μm. Source data are provided as a Source data file.

Article Snippet: For microinjection of DNA constructs, atub:UtrGFP, atub:GECO-RED, PACR (Addgene #55774), atub:PACR, CALM-GFP (Addgene #47602), CALMWT (Addgene #111499), CALM1234 (Addgene #111518).

Techniques: Expressing, Control, Activity Assay, Binding Assay, Mutagenesis, Injection